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To explore the mechanism of regulating reactive oxygen species (ROS)/Nod-like receptor family pyrin domain-contain-ning protein 3 (NLRP3) by Tilianin liposomes improved macrophage pyroptosis.
Macrophage pyroptosis model using lipopolysaccharide (LPS) combined with adenosine triphosphate (ATP) was established. They were divided into control group, model group, and experimental group (RAW 264.7 macrophages). The experimental group was treated with 5 μg·mL-1 Tilianin liposomes for 12 hours, while the control group and model group were cultured normally in complete culture medium without medication for 12 hours. Microplate method was used to detect the release of lactate dehydrogenase (LDH). Enzyme linked immunosorbent assay was used to detect the contents of interleukin (IL)-1β and IL-18. Detection of ROS content in cells was done by flow cytometry. Western blotting was used to detect the relative expression levels of NLRP3.
The LDH release levels in the experimental, control and model groups were (568.54±68.04), (556.05±83.31) and (984.41±138.82) U·L-1; the IL-1β contents were (8.99±2.17), (5.51±0.49) and (38.78±3.33) pg·mL-1; the IL-18 contents were (35.95±5.24), (20.95±2.36) and (64.14±9.73) pg·mL-1; the ROS release levels were 4.00±0.48, 0.68±0.50 and 9.58±0.42; the relative expression levels of NLRP3 protein were 2.44±0.46, 1.00±0.10 and 4.76±0.61, respectively. The above indicators in the experimental and control groups were compared with those in the model group, and there were statistically significant differences (all P<0.01).
Tilianin liposomes can reduce endogenous ROS production and improve LPS/ATP induced RAW 264.7 macrophage pyroptosis, which may be related to the regulation of ROS/NLRP3 inflammasome signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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