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To study the effects and safety of silica nanoparticles (SiNPs) on platelets and coagulation system, and initially to explore the mechanism related to the procoagulation of SiNPs.
Blood from the abdominal aorta of rats was taken for in vitro experiments to evaluate the blood safety of 10, 50, 100, 200 μg·mL-1 of SiNPs. Hematological toxicity was studied using hemolysis assay and lactate dehydrogenase assay. Platelet aggregation was detected by light transmission aggregometry. Platelet activation was detected by platelet surface activation receptor P-selectin. The effects of SiNPs on coagulation were also determined by activated partial tromboplastin time (APTT), thrombin time (TT), prothrombin time (PT) and fibrinogen (Fib).
In platelet-related studies, the max platelet aggregation was (49.38±13.66)% with SiNPs concentration of 200 μg·mL-1 and platelet activation occurred with 50 μg·mL-1 SiNPs exposing P-selectin. In coagulation sudies, the PT in 0.9% NaCl and 100 μg·mL-1 of SiNPs were (9.04±0.20) and (8.36±0.32) s, the blood coagulation indexes were (100.00±0.00)% and (90.06±7.81)%, and the differences were statistically significant (all P<0.05).
High concentrations of SiNPs can cause platelet aggregation and activation, shorten PT and promote blood coagulation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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