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To devolope a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of furmonertinib in human plsama and apply this method to analyze the blood concentration of non-small cell lung cancer (NSCLC) patients after taking medicine.
Furmonertinib -d3 was employed as the internal standard. The analyte and internal standard were extracted from plasma by protein precipitation with acetonitrile and chromatographed on Agilent Poroshell 120 SB-C18(2.1 mm×100.0 mm, 3.5 μm); mobile phase consisted of acetonitrile-10 mmoL-1 ammonium acetate(both of containing 0.1% formic acid); the whole analytical time was 3 min. The quantitative analysis were ionized with an electrospray ionization (ESI) source and operated in positive ion, multiple reaction monitoring (MRM) mode, the specificity, linear range, precision and accuracy, extraction recovery rate, matrix effect, dilution validation, and stability were all investigated.
The standard curves were demonstrated to be liner in the range of 0.50-100.00 μg·L-1 with y=3.95×10-2x-4.16×10-4 (r=0.995 4). The accuracy was 95.8%-107.3%. The coefficient of variation (CV) of inter-day (n=6) and intra-day (n=3) for four different concentration levels were less than 15%. The average recoveries and matrix effects were between 83.1%-88.2% and 94.6%-105.8%.
The established LC-MS/MS method for determining the concentration of vormetinib meets the requirements of biological sample analysis and can be used for the concentration determination of vormetinib in clinical practice.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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