To investigate the in vitro inhibitory effects of microRNA-28-5p (miR-28-5p) targeting G antigen 12I antibody (GAGE12I) on the proliferation, migration, and invasion of gastric cancer cells.

SGC-7901 cell were randomly divided into miR-28-5p group (miR-28-5p mimic), miR-NC group (miR-28-5p negative control mimic), shGAGE12I group (GAGE12I knockout) and blank group (GAGE12I not knockout). Cell proliferation, migration, and invasion capabilities were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and invasion chambers. Bioinformatics analysis and luciferase activity assays were conducted to examine the interaction between miR-28-5p and GAGE12I.

The proliferation rates of miR-28-5p and miR-NC groups were (99.90±10.30)% and (48.25±4.15)%; the invasion rates were (145.57±14.52)% and (67.30±6.95)%; the mobility rates were (103.58±10.75)% and (43.18±4.26)%, respectively, the differences were statistically significant (all P<0.05). The proliferation rates of shGAGE12I group and blank group were (99.80±10.20)% and (35.50±3.58)%; the invasion rates were (142.70±13.65)% and (65.30±6.75)%; the mobility rates were (103.55±10.06)% and (48.38±4.52)%, respectively, the differences were statistically significant (all P<0.05). GAGE12I was the target of miR-28-5p.

MiR-28-5p negatively regulates GAGE12I and reduces the proliferation, invasion and migration of gastric cancer cells.