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To study the effect of hydroxylsafflower yellow A (HSYA) on epiphyseal injury of tibial in rats through neurotrophic factor-3 (NT-3).
Fifty male SD rats were randomly divided into control group, model group, HSYA group, NC group and sh-NT-3 group, with 10 rats in each group. Except the control group, the injury model of tibial epiphyseal was established in the other 4 groups by high speed dental abrasive drill. The HSYA group was given 10 mg·kg-1 HSYA by intragastric administration once a day for 6 weeks. NC group and sh-NT-3 group were respectively injected with 1 mg·kg-1 sh-NC and sh-NT-3 through the tail vein, once every two days for 6 weeks. The control group and model group were given the same amount of normal saline intragastric and caudal intravenous injection. Quantitative real-time polymerase chain reaction was used to detect the expression level of NT-3 mRNA. The expression levels of bone repair markers and Notch receptor 1 (Notch1) signaling pathway related proteins were detected by Western blot.
The mRNA levels of NT-3 in control group, model group and HSYA group were 1.00±0.17, 3.31±0.59 and 5.16±0.84, respectively. The relative expression of runt related transcription factor 2 protein in control group, model group, HSYA group, NC group and sh-NT-3 group were 1.00±0.17, 3.67±0.63, 7.36±1.35, 7.31±1.42, 5.74±1.32, respectively; the relative expressions of osteoblast-specific transcription factor protein were 1.00±0.18, 5.76±1.01, 8.49±1.42, 8.56±1.54, 6.48±1.16, respectively; the relative expressions of Notch1 protein were 1.00±0.18, 1.44±0.23, 1.79±0.34, 1.83±0.35, 1.52±0.27, respectively. The above indexes in the model group were compared with the control group, the HSYA group was compared with the model group, and the sh-NT-3 group was compared with the NC group, and the above indexes were statistically significant (all P<0.05).
HSYA improves tibial epiphyseal injury in rats by promoting NT-3 expression and activating Notch1 signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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