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To explore the therapeutic effects and mechanisms of Dunhuang Xiexin decoction on mice with ulcerative colitis (UC).
Mice were randomly divided into blank group (0.9% NaCl), model group (0.9% NaCl) and experimental-L, -M, -H groups (2.93, 5.85, 11.70 g·kg-1 Dunhuang Xiexin decoction water extracts) with 10 cases per group for 7 days. Meanwhile, apart from the blank group, the other four groups of mice were allowed adlibitum access to drinking water containing 3% sodium dextran sulfate for 7 consecutive days to establish the UC model. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 in mouse serum were determined by enzyme-linked immunosorbent assay. The expression levels of occludin, zonula occludens-1 (ZO-1) and phospho nuclear factor kappa B p65 (p-NF-κB p65) in colonic tissues was detected by Western blot.
The serum TNF-α levels in the experimental- M, - H groups, model group and blank group were (397.57±33.05), (389.41±22.57), (472.19±18.70) and (355.92±31.86) pg·mL-1; the IL-1β levels were (73.06±18.38), (62.60±9.80), (98.94±27.50) and (55.26±18.14) ng·mL-1; the IL-6 levels were (153.23±32.96), (148.12±23.53), (239.35±50.99) and (131.36±27.58) pg·mL-1; the relative expressions levels of occludin protein in colon tissues were 0.80±0.13, 1.03±0.24, 0.57±0.15 and 1.10±0.03; the relative expressions levels of ZO-1 protein were 0.90±0.21, 1.09±0.10, 0.73±0.16 and 1.29±0.03; the relative expressions levels of p-NF-κB p65 protein were 0.75±0.12, 0.53±0.08, 1.15±0.19 and 0.43±0.21, respectively. There were significant differences between the experimental- H group and the model group (P<0.05).
Dunhuang Xiexin decoction may improve ulcerative colitis in mice by repairing the intestinal barrier and inhibiting the NF-κB signaling pathway to reduce the release of inflammatory factors.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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