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To investigate the mechanism of action of harmine (HM) in anti-Echinococcus granulosus (E.g) based on non-targeted metabolomics.
The aseptically cultured fine-grained E.g was divided into blank group (KB group, conventional culture), solvent group (DMSO group, DMSO 2 μL) and administration group (HM group, 20 mmoL·L-1 HM solution 2 μL). Survival rate was observed by eosin staining after 48 h of intervention and the ultrastructural changes were observed by scanning electron microscopy, respectively. Meanwhile, non-targeted metabolomics analysis was performed based on ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) technology on each group of E.g to screen the differential metabolites and explore the effect of harmine on the metabolic profile of E.g.
Eosin staining and scanning electron microscopy results showed that harmine had a significant inhibitory effect on E.g in vitro. Metabolomics results showed that there were a total of 15 metabolites with significant differences between the harmine and blank groups (all P<0.05). Enrichment of the differential metabolites by Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that harmine mainly affected the metabolic pathways of amino acid metabolism, fatty acid metabolism and nucleotide metabolism in E.g.
Harmine had a significant inhibitory effect on E.g, the mechanism of which may be related to the regulation of amino acid metabolism, fatty acid metabolism and nucleotide metabolism pathways, which can provide a new way of thinking for the development of the subsequent anti-cystic echinococlosis drug targets.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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