Full
Outline
In this study, dewatered sludge pre-granules were used as seed sludge to cultivate aerobic granular sludge (AGS) with high phosphorus removal capabilities over a 120-day operation. Inoculation with pre-granules allowed the system to bypass the initial granulation phase, establishing stable biological phosphorus removal by day 42. During the stable period, the experimental group achieved an average total phosphorus removal efficiency of (94.4±3.7)%, outperforming the control group (85.9±5.1)%. The pre-granulated AGS exhibited a particle size of 1100~1300μm, a sludge volume index of 50~80mL/g, and average removal efficiencies for chemical oxygen demand and total nitrogen of (94.5±2.9)% and (79.3±6.4)%, respectively. Scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS) revealed a notably higher iron content in the pre-granulated AGS (3.87% vs. 0.24% in the control), with iron uniformly distributed inside the granules. High-throughput sequencing further indicated enrichment of extracellular polymeric substance (EPS) secretion microorganisms, such as Candidatus_Competibacter (10.9% vs. 5.0% in the control). The results demonstrated that, unlike the primarily biological phosphorus removal mode in the control, the pre-granulated AGS developed a synergistic chemical-biological removal mechanism. Initially, EPS complexed with residual ferric iron from the dewatered sludge pre-granules, enhancing chemical phosphorus adsorption and providing a structural skeleton that improved granule stability. Subsequently, this stable structure facilitated microbial retention, yielding a higher mixed liquor volatile suspended solids concentration (4.3g/L vs.3.1g/L). Although the relative abundance of polyphosphate-accumulating organisms (PAOs) was similar between groups (0.48% vs. 0.47%), the absolute abundance of PAOs was elevated in the pre-granulated system. Moreover, the stable granular structure likely supported higher metabolic activity PAOs, as reflected by an increase in the relative abundance of key functional genes, such as polyphosphate kinase (ppk1, 0.42‰ vs. 0.34‰).
88
19
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
