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Degradation efficiency and pathways of oxytetracycline hydrochloride by Lysinibacillus sp.
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Jia-mei LI1, Zong-li KANG1, Ao-yu MA1, En-you ZHAO1, Hong-ling WANG2, Yu-hong YANG1, *, Li-yu DU3, *
China Environmental Science | 2026, 46(2) : 868 - 879
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China Environmental Science | 2026, 46(2): 868-879
• Soil Pollution Control •
Degradation efficiency and pathways of oxytetracycline hydrochloride by Lysinibacillus sp.
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Jia-mei LI1, Zong-li KANG1, Ao-yu MA1, En-you ZHAO1, Hong-ling WANG2, Yu-hong YANG1, *, Li-yu DU3, *
Affiliations
  • 1.College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China
  • 2.College of Chemical and Pharmaceutical Engineering, Shenyang University of Science and Technology, Shenyang 113122, China
  • 3.College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China
Published: 2026-02-20
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A highly efficient oxytetracycline hydrochloride (OTC-HCl)-degrading strain, designated as Lysinibacillus sp. OTC-L20, was isolated from contaminated soil. Its taxonomic status was confirmed via 16S rDNA sequencing, and it represent a highly efficient OTC-HCl-degrading strain within the genus Lysinibacillus. Genomic sequencing revealed that its chromosome harbors multiple antibiotic resistance genes, including those conferring resistance to tetracyclines and aminoglycosides, which may be involved in the biotransformation of OTC-HCl. Under specific culture conditions (supplemented carbon source at 4g/L, initial pH 8, inoculum size 3%, and initial substrate concentration 60mg/L), the strain achieved a degradation rate of 79.80% for OTC-HCl on the 7th day of incubation. Using ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS), a total of 14major intermediate metabolites were identified, and four distinct degradation pathways were proposed, involving seven reaction types such as deamidation and enol-keto isomerization. Compared with the blank control group, the degradation products of OTC-L20exhibited significantly reduced antibacterial toxicity to Escherichia coli and Staphylococcus aureus, demonstrating that Lysinibacillus sp. OTC-L20is a highly efficient and environmentally safe OTC-HCl-degrading bacterium.

oxytetracycline hydrochloride  /  Lysinibacillus subtilis  /  microbial degradation  /  degradation pathway
李佳美, 康宗利, 马骜雨, 赵恩佑, 王虹玲, 杨玉红, 杜立宇. 一株赖氨酸芽孢杆菌对盐酸土霉素降解效能及途径. 中国环境科学, 2026 , 46 (2) : 868 -879 .
Jia-mei LI, Zong-li KANG, Ao-yu MA, En-you ZHAO, Hong-ling WANG, Yu-hong YANG, Li-yu DU. Degradation efficiency and pathways of oxytetracycline hydrochloride by Lysinibacillus sp.[J]. China Environmental Science, 2026 , 46 (2) : 868 -879 .
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Year 2026 volume 46 Issue 2
72
12
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  • Receive Date：2025-07-16
  • Online Date：2026-10-08
  • Published：2026-02-20
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  • Received：2025-07-16
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Affiliations
    1.College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China
    2.College of Chemical and Pharmaceutical Engineering, Shenyang University of Science and Technology, Shenyang 113122, China
    3.College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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