To investigate the impact of the Water-Sediment Regulation Scheme (WSRS) on the size-fractionated distribution of phytoplankton in the coastal waters of the Yellow River Estuary, three surveys were conducted on June 24, July 6, and July 25, 2019. These surveys examined the spatial distribution of environmental factors, chlorophyll a (Chl a) concentrations, and phytoplankton size in both surface and bottom layers. The results indicated that the region consistently exhibited a nutrient structure characterized by high nitrogen and silica but low phosphorus. Throughout the survey period, nitrogen limitation was not observed in either the surface or bottom layers, while phosphorus limitation was persistent and intensified as the regulation progressed. During WSRS period, surface Chl a concentrations showed a decreasing trend followed by a gradual increase. In the pre-stage, high Chl a values were found near the river mouth, dominated by small-sized algae. During the water regulation stage (WR-stage), the high Chl a region expanded toward both sides of the estuarine mouth, with small-sized algae still dominant, though the proportion of microalgae notably decreased. In the sediment regulation stage (SR-stage), the high Chl a region shifted to the northeastern offshore area, with small-sized and picoplankton jointly dominating. In contrast, the Chl a concentration in bottom-layershowed an overall declining trend. During Pre and WR-stage, bottom Chl a levels were slightly lower than those in the surface layer, yet the spatial distribution and changes in size composition followed similar patterns. During SR-stage, high bottom Chl a concentrations were mainly found in the northeastern offshore area, where small-sized algae were significantly more dominant than in the surface layer. Redundancy analysis (RDA) revealed stage-specific shifts in the dominant environmental drivers. Before the WSRS, surface phytoplankton structure was influenced primarily by DIN/DIP, DSi/DIP, and DIP, while the bottom layer was controlled by DIN and salinity. During the WR-stage, DIN was the main driver in the surface layer, and DSi/DIN dominated the bottom. In the SR-stage, temperature, DIN, and DSi were key surface drivers, while temperature, DIN, and DIN/DIP governed bottom-layer variation.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |