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Graphene oxide-chitosan (GO-CS) composite materials were prepared using the hydrothermal method, and a single-stage autotrophic nitrogen removal reactor was constructed for continuous experiment. Batch experiments were conducted to evaluate its concentration-response characteristics, and molecular biology method was applied to elucidate the succession patterns of microbial communities. The effect of GO-CS addition on the performance of single-stage autotrophic nitrogen removal was systematically investigated. The results showed that 20mg/L GO-CS promoted anaerobic ammonium oxidation by facilitating electron transfer; at a concentration of 50mg/L, the activity of AnAOB was inhibited which might be there were other reactions which competing for the use of GO-CS as an electron transfer; and after long-term exposure to 100mg/L GO-CS, AnAOB achieved adaptive recovery by increasing the secretion of extracellular polymeric substances (EPS) and regulating functional genes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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