Surface and subsurface soils collected from a bamboo forest at the footslope of an upland were used to explore the effects of pig manure dissolved organic (PM DOM) and its hydrophilic (HI) and hydrophobic (HO) fractions on the adsorption, transport, and retention of doxycycline, enrofloxacin, florfenicol, and sulfamethazine. Total DOM was extracted from pig manure and further separated into HI and HO fractions using XAD-8resin column. According to the results of batch equilibrium experiments, the addition of PM DOM led to varying decreases in the adsorption of enrofloxacin, florfenicol, and sulfamethazine but marked increases in the adsorption of doxycycline; the addition of PM DOM's HIS fraction substantially weakened the adsorption of enrofloxacin and florfenicol by the soils; the addition of PM DOM's HO fraction significantly enhanced the adsorption of doxycycline but markedly reduced the adsorption of florfenicol by the soils. According to the results of saturated transport experiments in repacked soil columns, doxycycline and enrofloxacin could make a breakthrough; the HI fraction of PM DOM played a predominant role in reducing the recovery rate of sulfamethazine in the surface and subsurface soils (by 13.6% and 5.4%, respectively), while both the HI and HO fractions of PM DOM increased the recovery rate of florfenicol (only evidenced in the surface soil; by 30.7% and 20.1%, respectively). The addition of PM DOM could enhance the retention of sulfamethazine and florfenicol (by 14.9% and 7.8%, respectively, in the surface soil columns; by 3.1% and 10.6%, respectively, in the subsurface soil columns) while promote the redistribution of enrofloxacin and doxycycline to deeper soil layers. The humification degree and aromaticity of DOM in the aqueous phase of the soils, as well as the content and proportion of humic-like and protein-like components of DOM, might exert a crucial influence on the transport of veterinary drugs in the soils.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |