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Using ciprofloxacin (CIP) as a representative antibiotic, ciprofloxacin-resistant Serratia marcescens (CRSM) strains were isolated and characterized from the tertiary oxidation pond of an aquaculture wastewater treatment facility. The CRSM strains were systematically identified at the species level, and their resistance genes were comprehensively characterized using a third-generation sequencing platform. The inactivation efficiency was evaluated by examining the efficacy of Cu(Ⅲ) and hydroxyl radicals (·OH) generated by a Cu complex-activated PMS-based Fenton-like oxidation system on CRSM cells and genetic material in aqueous environments. The cytotoxic effects and molecular mechanisms of highly reactive oxidants in the homogeneous Cu-based Fenton-like oxidation system on CRSM were systematically investigated through a comprehensive analysis employing plate coating, scanning electron microscopy, transmission electron microscopy, and three-dimensional fluorescence spectroscopy. The results indicated that in the Cu(Ⅱ)+PMS+CIP+CRSM system, the bactericidal efficiency of CRSM was significantly enhanced, approximately 8-fold higher than that of the PMS+CIP+CRSM system. Specifically, the concentration of surviving bacteria decreased from 3.2×107CFU/mL to 1.5×102CFU/mL. The inactivation efficiency of CRSM reached approximately 97% within the initial 10-minutes, and exceeded 99.99% after a 30-minute treatment. Cu(Ⅲ) and ·OH achieved sterilization by compromising the integrity of CRSM cell membrane and cell wall, inducing damage to DNA bases (T, A, C, and G), inhibiting both intracellular and extracellular resistance genes, and degrading total DNA (both intracellular and extracellular).
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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