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Outline
In this study, 16commercial microbial samples were collected. Leveraging available phosphorus content as the screening criterion, we conducted preliminary screenings of phosphate-solubilizing efficiency in different microbial sources, optimized inoculation quantities, and developed composite microbial consortia through strain combination. Pot experiments were then carried out to evaluate the effects of tailings with composite microbial consortia on the growth and quality of Chinese cabbage. The results show that four efficient phosphorus-solubilizing bacteria were identified after delicately screening and then three composite bacterial agents (numbered as C3, C4 and C6) were obtained by orthogonal experiments. After cultivating for 7d, the phosphate-solubilizing efficiencies of these inoculants were increased by 7.1~7.6 times compared with that of comparison group. The pot experiment indicated that with phosphate tailings and composite microbial inoculant C4 were significantly improved in comparison group, that is the chlorophyll content and yield increased by 15.96% and 40.78%, respectively. Moreover, the contents of soluble protein and vitamin C in Chinese cabbage were significantly increased by 18.52% and 229.36%, respectively. In summary, the composite microbial inoculants significantly increased the available phosphorus content in phosphate tailings and demonstrated an important theoretical significance and a broad application potential in managing harmless treatment and efficient utilization of phosphate tailings.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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