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To address the resource utilization of agricultural wastes such as straw and livestock and poultry manure, this study investigated the effects of biochar addition on anaerobic digestion of a straw-manure mixture, using pig manure and wheat straw as raw materials. The biochar was devrived from the digestate obtained after anaerobic digestion of pig manure and wheat straw and its modification forms. The results showed that the addition of digestate-derived biochar played an important role in promoting electron transfer between species and providing more attachment sites for microorganisms. Specifically, biochar hydrolyzed with a manure-to- straw ratio of 1:1 and a solid content of 8% in the digestion system yielded the best gas production performance, increasing methane production by 17.59% compared to the control group without biochar. Furthermore, co-modification of this biochar with urea (as a nitrogen source) and nano zero-valent iron greatly enhanced its specific surface area and electron transfer capacity, leading to a 41.03% increase in methane production compared to the control. Additionally, the larger pore volume of digestate-derived biochar allowed it to adsorb volatile fatty acids from the digestate, thereby balancing the acidity and alkalinity of the system. The biochar also adsorbed up to 28.2% of ammonium nitrogen and 35.01% of nitrate nitrogen from the digestate, mitigating ammonium inhibition and potentially rectifying an imbalanced carbon-to-nitrogen ratio in the system. In conclusion, digestate-derived biochar and its modification represent an effective strategy for enhancing methane yield in anaerobic digestion systems.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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