To investigate the effect of fulvic acid (FA) addition on heavy metal speciation during aerobic composting of sludge-based biomass waste, a 44-day aerobic composting experiment was conducted using sludge, straw, and food waste as raw materials. The effects of FA at 100, 200, and 400mg·kg-1 on heavy metal transformation dynamics were examined. Results showed that FA addition increased peak temperature, cation exchange capacity (CEC), volatile solids (VS) degradation rate, and germination index (GI). FA notably facilitated the conversion of heavy metals into more stable residual fractions, with residual cobalt (RES-Co), nickel (RES-Ni), and copper (RES-Cu) contents increasing by 11.0%~16.9%, 4.1%~6.8%, and 0.8%~6.0%, respectively, compared to the control. By enhancing the accumulation of aromatic carbon in dissolved organic matter (DOM) and enriching specific microbial taxa (e.g., Proteobacteria and Bacteroidetes), FA accelerated humification and enhanced the immobilization of heavy metals. GI, CEC, pH, and RES-Ni were identified as key factors influencing microbial community shifts. The 400mg/kg FA treatment exhibited the most significant heavy metal passivation effect, primarily by accelerating the humification of dissolved organic matter and optimizing the microbial community structure.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |