This study investigated the effects of sugarcane bagasse biochar on the degradation of azoxystrobin and the structural composition of microbial communities in flooded paddy soils from South China using a microcosm system. Results demonstrated that biochar pyrolyzed at 300℃, 500℃, and 700℃ accelerated azoxystrobin degradation during the early incubation phase (10d), achieving degradation rates of 32.42%~34.87%, significantly higher than the 16.48% observed in the control (no biochar). However, it ultimately inhibited complete degradation by the late phase (50d), with final degradation rates of 88.78%~89.76% in biochar-amended soils versus 98.77% in the control, demonstrating an initial stimulation followed by suppression. The inhibitory effect intensified with increasing application rates of 700℃ biochar (BC700). Azoxystrobin acid, a key metabolite, was identified during this process. High-throughput 16S rRNA sequencing revealed that BC700 addition reduced total microbial species richness and Chao1index. Comparative analysis showed that 5% and 10% biochar treatments significantly decreased the relative abundance of Desulfobacterota while increasing Citrifermentans and Bacillus spp. compared to pristine soil and the 3% biochar treatment. Conversely, Clostridium_sensu_stricto_12 abundance declined.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |