On the basis of investigating the effect of fluoranthene (Flu) on the anaerobic co-digestion of sludge and kitchen waste, the mechanism on anaerobic co-digestion was comprehensively analysed. The results demonstrated that anaerobic methanogenesis was enhanced within the appropriate concentration of Flu (≤50mg/kg TSS). The maximum methane yield of the anaerobic co-digestion system [30.7mL/(gVSS·d)] was achieved at Flu concentration of 50mg/kg TSS, which was 1.61times that of the control group. Mechanistic studies showed that hydrolysis, acidification and acetoclastic methanogenesis were promoted by Flu, as evidenced by the increased activities of acetate kinase (ACK, 1.30-fold) and coenzyme F420 (1.43-fold) relative to the control. Microbial community structure demonstrated that not only Prevotella and Trichococcus, involved in organic matter hydrolysis and acidification, were selectively enriched in the Flu group, but also the relative abundance of the acetoclastic methanogen Methanosarcina was increased. Metagenomics further revealed that the relative abundances of functional genes associated with glycolysis (HK, pfk and PK), volatile fatty acid production (por, ackA and buk) and acetoclastic methanogenesis (pta, ACSS1_2, cdhD and cdhE) were up-regulated, favouring methanogenesis.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |