In this study, nitrate and ofloxacin(OFL) with high detection rate in groundwater were taken as target pollutants, and the effects of iron denitrification microorganism(M) with different inoculation dosage on the removal of nitrate and OFL by the dual materials of micro ZVI/FeS2 system was investigated. Also, the influence of the major water chemical parameters, nitrate and OFL concentrations on the removal of nitrate and OFL in the coupled system(ZVI/FeS2+M) was studied. In addition, the enhanced mechanisms for removing nitrate and OFL in the coupled system were explored. The results showed that the iron denitrification functional microorganisms could significantly enhance the removal of nitrate and OFL by ZVI/FeS2. When the mass ratio and total dosage of ZVI/FeS2 were 2:1 and 16g/L, respectively, and the inoculation amount of M was 5.81g/L, the nitrate in the ZVI/FeS2+M reaction system was almost completely removed within 168h, and the removal rate of OFL was as high as 90.44%. Meanwhile, the ZVI/FeS2+M system showed a good adaptability at different temperatures(10~30℃), nitrate concentrations(25~150mg/L), OFL concentrations(500ng/L~200ug/L) and the presence of coexisting ions. The intervention of microorganisms greatly delayed the passivation and oxidation of the ZVI surface, and at the same time, it could increase the dissolution of FeS2 to produce FeS, Fe2+ and S2-, which were all beneficial to the removal of pollutants. The removal of nitrate and OFL by ZVI/FeS2+M after six cycles was still kept at 73.80% and 79.25%, which was still higher than that of the ZVI/FeS2 (62.73% and 60.60%) in a one cycle experiment, indicating that the coupled system(ZVI/FeS2+M) is a feasible method for remediating nitrate and antibiotic-containing groundwater.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |