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When typical triphenylmethane dyes (crystal violet) and phenazine dyes (saffron T) were used as the research objects, the biotoxicity of dye wastewater was investigated before and after treatment using the Fe0/H2O2 process through the analysis in growth indicators (growth rate and inhibition rate), photosynthetic indicators (chlorophyll a, chlorophyll b, and carotenoid content), protein content, polysaccharide content, and antioxidant enzyme activity systems (superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA)) of Chlorella vulgaris (C.vulgaris). The results showed that with the extension of reaction time, the growth rate of C.vulgaris gradually decreased and its inhibition rate increased. The growth of C.vulgaris cells was significantly inhibited in raw dye wastewater. Compared with raw dye wastewater, chlorophyll-A content of C.vulgaris was increased by 22.01% in the effluent wastewater after treatment, and the contents of chlorophyll-B and carotenoid were decreased by 12.97% and 16.02%, respectively. The contents of SOD and CAT were increased by 15.13% and 12.5%, respectively, while the content of MDA was decreased by 23.97%, respectively. The protein content of C.vulgaris in raw dye wastewater was low, while its content was high in the effluent wastewater after treatment. Compared with raw dye wastewater, the content of polysaccharide in the effluent wastewater exhibited a decreasing trend. According to the toxicity prediction of algae using EPI Suite software, the toxicity of the intermediate product of crystal violet and saffron T degradation to fish, water flea and green algae was significantly reduced. Therefore, the biotoxicity of dye wastewater could be effectively reduced in the Fe0/H2O2 system.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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