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Outline
To explore the enhancement strategies for the methane production efficiency of anaerobic co-digestion systems by exogenous compound enzymes, corn stover and food waste were adopted as the mixed substrates. Subsequently, the combination and concentration of exogenous compound enzymes were optimized through batch experiments and the response surface methodology. In the single-factor experiment on lignin, the component and concentration range of the compound enzymes were screened. Moreover, 20groups of experiments with the concentrations of laccase, endo-β-1,4-glucanase and xylanase as variables were designed by means of the response surface methodology, and with the methane production promotion rate as the optimization objective. Based on the experimental data, a model was successfully established and variance analysis was carried out. The results showed that response surface analysis identified enzyme combinations within the ranges of laccase (1.5~4.0U/mL), xylanase (12~20U/mL) and endo-β-1,4-glucanase (50~85U/mL), which could achieve a high methane production promotion rate, was finally determined as the appropriate component of the compound enzyme. The continuous flow test validated the efficacy of the composite enzyme, indicating that the addition of the compound enzyme promoted the proliferation of hydrolases and aceticlastic methanogens, released lignin, cellulase and anaerobic digestion enzymes with higher activities, and thus increased the methane production rate of the anaerobic co-digestion system by 19.5%.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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