Targeting the challenges in organic micropollutants (OMPs) detection and biotoxicity assessment, this study systematically evaluated four water pretreatment approaches and seven activated elution solvent combinations to improve the enrichment efficiency of residual organic matter in reclaimed water. The optimized procedure comprised: acidification to pH 2.5 with 5% (V/V) methanol addition; sequential SPE using HLB (500mg/6mL) and LC-18 (500mg/6mL) cartridges for broad-spectrum retention; ternary elution with 10mL methanol, 10mL ethyl acetate, and hexane (1:1V/V) to achieve polarity-balanced desorption. The optimized approach achieved 88% dissolved organic carbon (DOC) recovery, representing a 1.3~15.9-fold enhancement in OMPs recovery across compounds with logKow values ranging from 1.8 to 5.7 compared to conventional methanol-based methods. Notably, the optimized protocol reduced the sample volume required to reach EC50 in acute toxicity assays by 4.4-fold. The optimized method developed in this study significantly enhanced detection sensitivity and precision.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |