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A heterotrophic nitrifying-aerobic denitrifying bacterium, identified as Thauera butanivorans SVT, was isolated from spice wastewater. Genomic DNA of the isolate was extracted for whole-genome sequencing to analyze and predict nitrogen metabolic pathways. The nitrogen metabolism characteristics were investigated using 100mg/L NH4+-N, NO3--N, or NO2--N as sole nitrogen sources. The effects of different carbon sources (sodium acetate anhydrous, sodium citrate, sodium succinate, sucrose, and glucose), C/N ratios (1, 5, 10, 15, 30), temperatures (10℃, 20℃, 30℃, 40℃, 50℃), and pH levels (4.0, 6.5, 7.5, 8.5, 10.0) on its heterotrophic nitrification-aerobic denitrification performance were systematically investigated. Results showed total nitrogen removal rates of (86.96±1.03)%, (60.52±4.70)%, and (64.32±1.40)% within 48hours when using NH4+-N, NO3--N, and NO2--N as sole nitrogen sources, respectively. Strain SVT demonstrated growth and nitrogen removal capabilities under all tested conditions, with optimal performance achieved using sodium acetate anhydrous as carbon source at 30℃, C/N=10, and pH 7.5. Genomic analysis identified six nitrogen metabolism-related functional enzyme genes (napAB, nxrAB, nirBD, nirS, norBC, and nosZ). Combined with KEGG database alignment and single nitrogen source metabolism results, the proposed nitrogen metabolic pathways include: ammonium assimilation (NH4+-N→L-glutamate→L-glutamine), nitrification (NH4+-N→NO2--N→NO3--N), denitrification (NO3--N→NO2--N→NO→N2O→N2), and DNRA pathway (NO3--N→NO2--N→NH4+-N). This study demonstrates that strain SVT exhibits efficient heterotrophic nitrification-aerobic denitrification capabilities, showing promising potential for wastewater treatment applications.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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