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Unveiling the mechanism for the rapid development of hydrostatically oxygenic photogranules
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Yu-chen AN, Bing ZHANG, Jia-wei FAN, Wen-xin SHI*
China Environmental Science | 2025, 45(7) : 3714 - 3723
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China Environmental Science | 2025, 45(7): 3714-3723
• Water Pollution Control •
Unveiling the mechanism for the rapid development of hydrostatically oxygenic photogranules
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Yu-chen AN, Bing ZHANG, Jia-wei FAN, Wen-xin SHI*
Affiliations
  • Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400045, China
Published: 2025-07-20
Outline
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In order to strengthen the rapid and stable formation of hydrostatically oxygenic photogranules (HSP), a strategy of inoculating Oscillatoria sp., a typical kind of filamentous cyanobacteria, into activated sludge at the early stage of cultivation was proposed. The photogranulation process, morphology, extracellular polymeric substances contents, surface thermodynamics properties and microbial community were analyzed to elucidate the mechanism for efficient photogranulation in R1 (cultivated with Oscillatoria sp.). Results showed that the mixed liquid suspended solid of mature HSP in R1 (2.17g/L) was larger than that in R2 (cultivated without Oscillatoria sp.) (2.03g/L), and the surface of HSP in R1 was smoother. Values of PN/PS in R1 (2.01) was slightly larger than R2 (1.45) on 30d, implying that inoculating Oscillatoria sp. enhanced the hydrophobicity of granular sludge. Furthermore, results showed that the interfacial free energy was strengthened in R1 with the potential energy barrier of 2616.75kT, which was lower than the potential energy barrier in R2 (5037.85kT). The EPS producing bacteria were enriched and the relative abundance of enzymes related to EPS production was increased by 8%~156% in HSP after being strengthened by Oscillatoria sp. Overall, inoculating filamentous cyanobacteria Oscillatoria sp. into activated sludge facilitated the attachment and aggregation between bacteria and microalgae in HSP, contributing to the rapid development of HSP.

algal-bacterial symbiotic  /  strengthening strategy  /  formation mechanism  /  hydrophilicity and hydrophobicity  /  extended Derjaguin-Landau-Verwey-Overbeek theory
安宇晨, 张冰, 樊佳炜, 时文歆. 静态产氧光颗粒的快速形成过程及自凝聚机理. 中国环境科学, 2025 , 45 (7) : 3714 -3723 .
Yu-chen AN, Bing ZHANG, Jia-wei FAN, Wen-xin SHI. Unveiling the mechanism for the rapid development of hydrostatically oxygenic photogranules[J]. China Environmental Science, 2025 , 45 (7) : 3714 -3723 .
Year 2025 volume 45 Issue 7
60
18
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Article Info
  • Receive Date:2024-12-17
  • Online Date:2026-10-08
  • Published:2025-07-20
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  • Received:2024-12-17
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    Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400045, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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