In order to strengthen the rapid and stable formation of hydrostatically oxygenic photogranules (HSP), a strategy of inoculating Oscillatoria sp., a typical kind of filamentous cyanobacteria, into activated sludge at the early stage of cultivation was proposed. The photogranulation process, morphology, extracellular polymeric substances contents, surface thermodynamics properties and microbial community were analyzed to elucidate the mechanism for efficient photogranulation in R1 (cultivated with Oscillatoria sp.). Results showed that the mixed liquid suspended solid of mature HSP in R1 (2.17g/L) was larger than that in R2 (cultivated without Oscillatoria sp.) (2.03g/L), and the surface of HSP in R1 was smoother. Values of PN/PS in R1 (2.01) was slightly larger than R2 (1.45) on 30d, implying that inoculating Oscillatoria sp. enhanced the hydrophobicity of granular sludge. Furthermore, results showed that the interfacial free energy was strengthened in R1 with the potential energy barrier of 2616.75kT, which was lower than the potential energy barrier in R2 (5037.85kT). The EPS producing bacteria were enriched and the relative abundance of enzymes related to EPS production was increased by 8%~156% in HSP after being strengthened by Oscillatoria sp. Overall, inoculating filamentous cyanobacteria Oscillatoria sp. into activated sludge facilitated the attachment and aggregation between bacteria and microalgae in HSP, contributing to the rapid development of HSP.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |