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In order to address the issues of complex processes and high infrastructure and operational costs in simultaneous nitrogen and phosphorus removal, a heterotrophic nitrifying strain NP3 exhibiting simultaneous nitrogen and phosphorus removal capabilities was isolated from activated sludge in this study. Strain NP3 was identified as Pseudomonas stutzeri by 16S rRNA sequence analysis, and its nitrogen and phosphorus removal characteristics and mechanisms were investigated. It was showed that strain NP3 was able to utilize ammonium, nitrate, and nitrite as the sole nitrogen source for efficient nitrogen and phosphorus removal under aerobic conditions. The accumulation of intermediate products during the reaction process was minimal, and nitrogen and phosphorus were primarily removed through assimilation. The growth and metabolic rates followed the order: NH4+-N >NO2−-N >NO3−-N. Under the optimal growth conditions of sodium citrate as the carbon source, C/N=10, T=30℃, pH =7, and r=160r/min, the maximum removal rates of ammonia nitrogen and phosphate were almost 100%. Furthermore, successful amplification of denitrification and polyphosphate genes (nosZ, nirS, ppk) further confirmed the simultaneous nitrogen and phosphorus removal capability of strain NP3. X-ray Photoelectron Spectroscopy (XPS) analysis demonstrated that the functional groups on the extracellular polymeric substances(EPS) surface could adsorb different forms of phosphorus such as C-PO3/P-C, PO43-/HPO42-, acting as phosphorus transfer stations.31P nuclear magnetic resonance (NMR) results further indicated that there was a large effect of EPS on phosphorus fugitive morphology, with pyrophosphate being the main phosphorus species in the presence of EPS, whereas orthophosphate and orthophosphate diester were the major phosphorus forms after EPS extraction.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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