In order to explore the community structure and construction mechanism of soil protozoa in Lhamu Lhacuo National Wetland Park, 24 large samples were set up in July 2023 according to the geographical and habitat characteristics in the study using the plum blossom five-point sampling method with a total of 120 soil samples. The culture and morphological identification of soil protozoa were performed by non-submerged culture method and in vivo observation method. The quantitative study was conducted by Petri dish direct counting method. A total of 175 soil protozoa species were identified, belonging to 12 classes, 29 orders, 55 families and 91 genera, mainly composed by species of Zoomastigophorea accounting for 15.43% of the total species, and species of Heliozoea was the least, accounting for 1.14% of the total species only. The α diversity of soil protozoa community in Lhamu Lhacuo National Wetland Park. had no significant difference between slopes(P>0.05), and the Shannon-Wiener diversity index and Simpson dominance index at low, middle and high altitudes were significantly different from those at high altitude(P<0.05). In the co-occurrence network analysis, the relationships between soil protozoan communities in different slope aspects and at different altitudes are mainly positively correlated. Altitude, pH value, soil water content, soil organic matter, available potassium, and vegetation coverage are the main soil environmental factors that affect the composition of soil protozoan communities. The results of this study are helpful for evaluating and monitoring the changes in the soil environmental quality of the Lhamu Lhacuo National Wetland Park, and can provide basic theoretical data for the protection of soil ecological functions and the sustainable development in the Tibet region.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |