In view of the typically unsatisfactory antibiotics removal performances that were observed in the traditional 'three ponds and two dams' combination process, a new composite packing filter dam was developed. Through the synergetic combination of composite packing balls with a specially designed filter dam structure, highly efficient and broad-spectrum removal of antibiotics was achieved. Results showed that the removal rates of antibiotics (in terms of total mass concentrations) in perch, eel, raw fish and shrimp culture pond water were maintained at more than 80% by the composite packing filter dam. Quinolones, sulphonamides, tetracyclines and chloramphenicol were removed to different extents, among which the best removal effects were observed for quinolones and sulphonamides. The composite filler consisting of iron filings, ceramsites and polybutylene succinate (PBS) was found to significantly improve the removal of quinolones and sulfonamides. Ceramsites were demonstrated to play an adsorption role through which quinolone and sulfonamide antibiotics were removed via pore filling and π-π electron donor-acceptor interactions, which was identified as the main antibiotic removal pathway. Iron filings were shown to remove tetracycline and chloramphenicol through adsorption and reduction processes, and were suggested to have accelerated the direct electron transfer process that promoted antibiotic degradation. PBS was involved in the removal of antibiotics through co-metabolic denitrification. Both iron filings and PBS were proven to enhance the metabolic activity of functional microorganisms, thereby accelerating antibiotic removal. The synergistic effect between these components was confirmed to help achieve efficient and broad-spectrum antibiotic removal.