This study takes the Wujing Road Tunnel in Tianjin as an example to explore the emission characteristics of benzothiazoles (BTs) in particulate, gaseous pollutants, and road dust. The results show that the concentrations of particulate matter, gaseous pollutants, and BTs in road dust exhibit regular patterns, especially the concentration changes of 2-hydroxybenzothiazole (2-OH-BT) and benzothiazole (BT). Since BTs in the enclosed tunnel environment mainly originate from tire wear particles of motor vehicles, 2-OH-BT and BT can serve as important tracers for identifying non-exhaust emissions from motor vehicles. During the tunnel experiment, the daily traffic volume ranged from 11,972 to 16, 157 vehicles per day, the total carbon (TC) concentration was between 10.85and 15.75μg/m³, and the BTs concentration was between 3.33 and 8.41ng/m³. The gas-particle ratio values of 2-mercaptobenzothiazole (MBT), 2-OH-BT, and BT in the tunnel were generally higher than those in the receptor environment, and 2-OH-BT and BT were the dominant gaseous BTs components. This indicates that most MBT, 2-OH-BT, and BT generated from tire wear sources of motor vehicles are released in the gaseous phase, so the gaseous BTs should not be overlooked. For the calculation of motor vehicle emission factors, the average emission factors of organic carbon (OC), elemental carbon (EC), and PM_2.5 in the Wujing Road Tunnel were 2.80, 1.60, and 13.77mg/(km⋅vehicle), respectively. In the health risk assessment model, the daily exposure to BTs through ingestion was the highest. The daily intake for children and adults was 12.03 and 1.29ng/(kg⋅d), respectively. The total daily exposure for children was more than nine times that of adults, indicating that children may face a greater health threat from traffic pollution than adults.