Full
Outline
To rapidly screen natural antibacterial active components targeting lipopolysaccharide (LPS) through the detection of traditional Chinese medicine (TCM) monomers via plasmonic near-field sensing. In this study, a plasmonic near-field biosensor based on gold nanoislands (AuNIs) and LPS (LPS@AuNIs biosensor) was constructed, and the LPS antagonistic activity of natural products derived from TCM was evaluated by the change in phase response. The limit of detection (LOD) and limit of quantification (LOQ) of the LPS@AuNIs biosensor for polymyxin B (PB), which specifically binds to LPS, were 6.34 ± 0.002 374 and 61.7 ± 0.001 735 nmol·L-1, respectively. Detection results of 14 TCM monomers showed that the LPS@AuNIs biosensor exhibited excellent concentration-dependent responses to both 1, 2, 3, 4, 6-O-pentagalloylglucose (β-PGG) and geniposide (GP). Comparative analysis revealed that β-PGG displayed potent binding activity, with a dissociation constant (ka) superior to that of PB. Further verification via minimum inhibitory concentration (MIC) and displacement assays confirmed that β-PGG possessed strong binding affinity comparable to PB. The developed biosensor enables specific binding between LPS and analytes, featuring high sensitivity and detection efficiency for high-precision quantitative analysis. This study innovatively integrates nanophotonics with natural medicine research, establishing an integrated system of "drug screening-target validation" and providing a high-efficiency technical platform for the rapid and effective screening of natural antibacterial active components.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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