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Due to the inability of the current quality standards to effectively control the quality of Lepidii Semen, this study established the HPLC characteristic chromatogram and multi-component content determination method for Lepidii Semen. Using a NanoChrom ChromCore HP AQ C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile and 0.2% phosphoric acid aqueous solution as the mobile phase for gradient elution at a flow rate of 1.0 mL·min-1, with a column temperature of 35 ℃, and detection wavelength of 210 nm. The characteristic HPLC chromatogram of Lepidii Semen was established with twelve characteristic peaks marked, eleven of which were identified by comparison with reference standards. The similarity of 13 batches of market-sourced samples exceeded 0.90. The established chromatogram effectively distinguished Lepidii Semen from four other cruciferous seeds and four common adulterants. The method was simultaneously applied to determine the contents of glucotropaeolin (GPL), glucoerucin (GR), sinapine thiocyanate (SPT), and quercetin-3-O-β-D-[2-O-(6-O-sinapoyl)-β-D-glucopyranosyl]-glucopyranoside (QSGG). Two detection wavelengths (210 and 330 nm) were selected based on the maximum absorption of each compound. Method validation confirmed that the method possesses high specificity, good linearity, and satisfactory precision, repeatability, stability, and recovery rates. The contents range of GPL, GR, SPT and QSGG in 13 batches of Lepidii Semen were 1.58%-2.40%, 1.27%-1.78%, 0.22%-0.27%, and 0.28%-0.42%, respectively. The established HPLC characteristic chromatogram and multi-component content determination methods are stable and reliable, providing a scientific basis for the quality control and evaluation of Lepidii Semen, standardization of the herbal market and rational clinical use.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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