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A systematic phytochemical investigation of Polygonatum cyrtonema Hua. processed by nine cycles of steaming and sun-drying was carried out using silica gel, Toyopearl HW-40C, octadecylsilyl (ODS) silica gel, and semi-preparative HPLC, leading to the isolation of three nitrogen-containing compounds. The structures of the isolates were elucidated by comprehensive spectroscopic analyses, including HRESIMS, NMR, IR, and UV, and were identified as cyrtoneine F (1), (2S,5S)-5-[(1S)-1-methylpropyl]-3,6-dioxo-2-piperazine propanoic acid (2), (2S,5S)-5-(2-methylpropyl)-3,6-dioxo-2-piperazine propanoic acid (3). Compound 1 was characterized as a new amide, whereas compounds 2 and 3 were determined to be diketopiperazines, which were isolated for the first time from Polygonatum cyrtonema Hua.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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