Full
Outline
Drought is a critical stress factor severely restricting the growth and development of Thesium chinense Turcz., while the WOX (WUSCHEL-related homeobox) transcription factor family plays an important role in plant growth, development, and stress response. To explore the drought resistance mechanisms of T. chinense and identify relevant WOX genes, this study simulated drought stress using 20% PEG6000, combined with physiological and transcriptomic analyses to reveal its response strategies. The results indicated that drought stress significantly inhibited plant growth and photosynthesis but activated the antioxidant enzyme system (with CAT, POD, SOD, and APX activities reaching 4.09, 1.57, 3.05, and 58.44 times those of the control, respectively). Additionally, it promoted the accumulation of secondary metabolites such as total polysaccharides, total flavonoids, and thesiumine Ⅰ. Through transcriptomic analysis, the candidate gene TcWOX8 was identified. TcWOX8 was successfully cloned from T. chinense, with a full length of 897 bp encoding 298 amino acids, a protein molecular mass of 33.6 kD, and containing a conserved Homeodomain. Phylogenetic analysis revealed its closest relationship to WOX8 from Vitis vinifera and Hordeum vulgare, suggesting its potential involvement in plant abiotic stress responses. Further experiments confirmed that the TcWOX8 protein can be solubly expressed, possesses transcriptional activation activity, and localizes to the nucleus. The expression of this gene was not only induced by drought stress but also significantly responded to salt, low-temperature, and high-temperature stresses. These results provide a solid molecular foundation for elucidating the drought resistance mechanisms of T. chinense and revealing the key regulatory role of TcWOX8 in broad-spectrum stress defense in plants.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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