Full
Outline
Polymer-drug conjugates have shown great potential in the field of drug delivery, but existing systemic pharmacokinetic data are difficult to clarify their pharmacokinetic behavior at the cellular level. In this study, multi-loaded pegylated irinotecan conjugate (PEG-[irinotecan]3) was used as a model drug to build a research idea based on the interaction of polymorphic components to analyze its cellular pharmacokinetics. An HPLC-MS/MS analysis method based on in-source collision-induced dissociation (IS-CID) technology was established to achieve accurate quantitative analysis of PEG-[irinotecan]3 and its intracellular polymorphic components, and comprehensively clarify its intracellular uptake, efflux and subcellular distribution characteristics. Uptake studies have shown the presence of polymorphic components in cells such as bonded drugs (PEG-[irinotecan]x (x = 3, 2, 1)), release material (PEG20K), free drug (irinotecan) and active metabolite (SN-38). Subcellular distribution studies have shown that bonded drugs and release materials are mainly distributed in the cytoplasm, while irinotecan is mainly distributed in the cytoplasm and nucleus. This study expands the cellular pharmacokinetic research system of polymer-drug conjugates, and the key information obtained is of great value in guiding drug design, clarifying intracellular pharmacokinetic behavior, and promoting clinical transformation.
176
93
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
