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To explore the reasons for the uneven quality of commercial Eucommia ulmoides leaves, an HPLC method based on deep eutectic solvent (DES) and microwave-assisted extraction (MAE) was developed to determine the contents of eucommiol, aucubin, geniposidic acid, chlorogenic acid and asperuloside. A DES composed of choline chloride and urea (molar ratio 1∶2) with water content of 40% exhibited the highest efficacy. MAE was selected and the optimized conditions were microwave power of 70 W and extraction time of 15 s. The contents of five compounds in green leaves were significantly higher than those in yellow-brown leaves. Part of yellow-brown leaves even lacked aucubin, geniposidic acid and asperuloside. The contents of five compounds were the highest in the leaves treated with sun-drying and low-temperature drying, followed by the deactivated enzymes leaves and shade-drying leaves. The content of each chemical component, especially iridoids, decreased significantly in room temperature. However, the contents of the compounds decreased slowly in low temperature. There was a significant difference in the quality of commercially available green and yellow-brown Eucommia ulmoides leaves, which may be related to the drying and storage methods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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