Full
Outline
In this study, LHCGR(-Ex10)-CRE-luc-HEK293 cell line and LHCGR-CRE-luc-HEK293 cell line were constructed according to the cAMP signaling pathway activated by luteinizing hormone (LH) binding to the receptor. The biological activity of LH was detected by luciferase detection system, and the relative titer of samples was calculated by four parameter fitting analysis. The method conditions were optimized, and the specificity, relative accuracy, precision and linearity of the method were verified. The results showed that there was a dose-response relationship between the concentration of LH and the expression of reporter gene, and it conformed to the four-parameter curve. After optimization, the conditions were determined as follows: the initial concentration of LH was 200 nmol·mL-1, 6-fold serial dilution, 10 concentrations, the number of cells per well was 30 000, and the incubation time was 6 h. It is proved that the method has strong specificity, good accuracy, precision and linearity. This study successfully established and validated a reporter gene method to detect LH biological activity, which can be used for LH biological activity detection and quality control. With the increasing demand for the research and development of LH biosimilar drugs, the establishment of a stable and simple activity assay method to evaluate the biological activity of LH can provide technical support for the quality control of LH products and a powerful tool for the comparative study of similar products.
252
125
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
