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Nineteen compounds were isolated from the ethyl acetate fraction of the stems and leaves of Salvia miltiorrhiza Bge by using various column chromatographic methods, such as silica gel, Sephadex LH-20 and semi-preparative HPLC. According to their physiochemical properties and spectral data, the structures were further identified as 3′,4′-dihydroxy-4-(3-methoxy-3-oxopropyl)-[1,1′-biphenyl]-3-carboxylic acid (1), 2-hydroxy-5-(2′-hydroxybenzyl) benzoic acid (2), dehydrozingerone (3), 3,4-dihydroxy benzalacetone (4), benzyl alcohol-β-D-glucopyranoside (5), dehydrololiolide (6), rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo[3,2,1]-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid (7), 6-hydroxy-3-(2-hydroxyethyl)-2,4,4-trimethylcy clohexa-2,5-dien-1-one (8), scopoletin (9), esculetin (10), dihydrotanshinone Ⅰ (11), 2′-deoxythymidine (12), quercetin 3-glucoside (13), sutchuenmedin A (14), 2′′-O-rhamnosylicariside-Ⅱ (15), 2,2′-oxybis(1,4-di-tert-butylbenzene) (16), 1H-indole-3-carbaldehyde (17), 3-(2-oxopropyl)-3-hydroxyind-olin-2-one (18), dysodensiol E (19). Compound 1 was a new biphenyl lignan, named as 3′,4′-dihydroxy-4-(3-methoxy-3-oxopropyl)-[1,1′-biphenyl]-3-carboxylic acid. Compound 2 was a new natural product and compounds 3-8 and 4-19 were firstly isolated from S. miltiorrhiza. MTT method was used to screen the anti-hepatoma activity of all the compounds, and none of them showed obvious effect.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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