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The anti-ZIKA virus activity of tenofovir disoproxil fumarate in vitro
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Xiu-xiu CHEN1, 2, 4, Rong-hua LUO2, Chang-bo ZHENG3, Zhai-wen YAO1, 2, 4, Qiu-ju TANG3, Si-dong XIONG4, Yong-tang ZHENG2, 4, *
Acta Pharmaceutica Sinica | 2019, 54(9) : 1582 - 1587
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Acta Pharmaceutica Sinica | 2019, 54(9): 1582-1587
• Original Articles •
The anti-ZIKA virus activity of tenofovir disoproxil fumarate in vitro
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Xiu-xiu CHEN1, 2, 4, Rong-hua LUO2, Chang-bo ZHENG3, Zhai-wen YAO1, 2, 4, Qiu-ju TANG3, Si-dong XIONG4, Yong-tang ZHENG2, 4, *
Affiliations
  • 1. College of Pharmacy, Soochow University, Suzhou 215021, China
  • 2. Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
  • 3. School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, China
  • 4. KIZ-SU Joint Laboratory of Animal Model and Drug Development, College of Pharmaceutical Sciences, Soochow University, Suzhou 215021, China
Published: 2019-09-12 doi: 10.16438/j.0513-4870.2018-1135
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Tenofovir disoproxil fumarate (TDF) is a nucleoside analogue that has been widely used for clinical treatment of human immunodeficiency virus (HIV) and hepatitis B virus (HBV) infection. The aim of this study was to investigate whether TDF has anti-Zika virus (ZIKV) activity in vitro. The inhibitory effect of TDF on ZIKV was detected by plaque reduction assay. Then, the anti-ZIKV activity of TDF at RNA level and protein level was verified by real time quantitative PCR and Western blot. Finally, MTT assay was used to determine the cytotoxicity of TDF. Our results showed that TDF not only reduced the formation of plaque after ZIKV infection, but also inhibited the replication of ZIKV RNA or expression of ZIKV NS2B protein. The 50% effective concentration (EC50) of TDF in inhibition of ZIKV replication were 14.96-27.47 μmol·L-1, while that of ribavirin was 56.01 ±12.16 μmol·L-1, which served as the positive control. The cytotoxicity of TDF and ribavirin in Vero cells were very low, with their 50% cytotoxic concentration (CC50) values being greater than 500 μmol·L-1. The therapeutic index of TDF calculated by CC50/EC50 was greater than 18.20, which was significantly higher than that of ribavirin. The results suggest that TDF has good anti-ZIKV activity in vitro and is expected to become a candidate drug for anti-ZIKV therapy.

tenofovir disoproxil fumarate  /  nucleoside analogue  /  Zika virus  /  antiviral activity  /  therapy
Xiu-xiu CHEN, Rong-hua LUO, Chang-bo ZHENG, Zhai-wen YAO, Qiu-ju TANG, Si-dong XIONG, Yong-tang ZHENG. The anti-ZIKA virus activity of tenofovir disoproxil fumarate in vitro[J]. Acta Pharmaceutica Sinica, 2019 , 54 (9) : 1582 -1587 . DOI: 10.16438/j.0513-4870.2018-1135
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Article Info
doi: 10.16438/j.0513-4870.2018-1135
  • Receive Date：2018-12-23
  • Online Date：2026-01-26
  • Published：2019-09-12
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History
  • Received：2018-12-23
  • Revised：2019-01-08
Funding
Affiliations
    1. College of Pharmacy, Soochow University, Suzhou 215021, China
    2. Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
    3. School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, China
    4. KIZ-SU Joint Laboratory of Animal Model and Drug Development, College of Pharmaceutical Sciences, Soochow University, Suzhou 215021, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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