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Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography
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Chen SUN1, 2, Zhe-yu XIE2, Yong-jiang WU1, *
Acta Pharmaceutica Sinica | 2019, 54(11) : 2059 - 2063
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Acta Pharmaceutica Sinica | 2019, 54(11): 2059-2063
• Special Reports •
Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography
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Chen SUN1, 2, Zhe-yu XIE2, Yong-jiang WU1, *
Affiliations
  • 1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
  • 2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China
Published: 2019-11-12 doi: 10.16438/j.0513-4870.2019-0407
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An HPLC fingerprint and multi-component determination method of Leonurus japonicus was established for comprehensive evaluation and quality control of Leonurus japonicus. The sample was incubated in 70% ethanol in a water bath for 2 h, and the extract was analyzed by HPLC using Kromasil C18 column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min-1. The temperature of column was 30℃. The detection wavelength was 280 nm. HPLC fingerprint of characteristic components of Leonurus japonicus was established. There were 12 common peaks among 25 batches of samples, and 5 of them were identified and determined. Syringic acid, leonurine hydrochloride, rutin, hyperoside or isoquercitrin showed a good linearity in the ranges of 0.426 1-85.22 ng (r=0.999 9), 7.948-1 590 ng (r=0.999 3), 10.20-2 040 ng (r=1.000 0), 2.018-403.6 ng (r=0.999 9), or 8.704-1 741 ng (r=0.999 9), respectively. The average recoveries were 99.0%, 97.6%, 97.4%, 96.9% and 98.5% with RSD of 1.1%, 1.8%, 1.4%, 1.5% and 1.3%, respectively. The HPLC characteristic fingerprint of Leonurus japonicus was specific, and this method can simultaneously determine the content of 5 components.

Leonurus japonicus  /  fingerprint  /  multi-component determination
Chen SUN, Zhe-yu XIE, Yong-jiang WU. Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography[J]. Acta Pharmaceutica Sinica, 2019 , 54 (11) : 2059 -2063 . DOI: 10.16438/j.0513-4870.2019-0407
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Article Info
doi: 10.16438/j.0513-4870.2019-0407
  • Receive Date：2019-05-24
  • Online Date：2026-01-26
  • Published：2019-11-12
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  • Received：2019-05-24
  • Revised：2019-07-04
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    1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
    2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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