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The effects of different stimulators on the activation of human lymphocytes
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Zheng YAN, Ni-na XUE, Ming JI, Fang-fang LAI, Xiao-guang CHEN*
Acta Pharmaceutica Sinica | 2019, 54(10) : 1863 - 1867
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Acta Pharmaceutica Sinica | 2019, 54(10): 1863-1867
• Original Articles •
The effects of different stimulators on the activation of human lymphocytes
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Zheng YAN, Ni-na XUE, Ming JI, Fang-fang LAI, Xiao-guang CHEN*
Affiliations
  • State Key Laboratory of Bioactive Substances and Functions of Natural Medicines/Beijing Key Laboratory of Non-clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Published: 2019-10-12 doi: 10.16438/j.0513-4870.2019-0670
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The research aims to study the effects of different stimulants on the activation of human lymphocytes. Human peripheral blood mononuclear cells were prepared by density centrifugation. The blood's sample was provided by National Institutes for Food and Drug Control and approved by its Ethics Committee. The expressions of CD69 in CD3+CD4+ and CD3+CD8+ human T cells were detected by flow cytometry after administrated with CD3/CD28 antibody, phytohaemagglutinin (PHA), Staphylococcus auresus enterooxin B (SEB), interleukin (IL27) and PMA plus ionomycin for 24 h. The proliferation of lymphocyte was detected by CellTiter-Glo kit. The secreted IFNγ in supernatant of medium was examined by ELISA kit. The proliferation of lymphocytes had no change after exposed of CD3/CD28 antibody, SEB, IL27 and PMA plus ionomycin for 24 h. However, the CD69 expressions in CD3+CD4+ and CD3+CD8+ T cells and IFNγ productions were significantly increased by CD3/CD28 antibody, SEB, IL27 and PMA plus ionomycin at 24 h, indicating that CD3+CD4+ and CD3+CD8+ T cells were activated under above-mentioned stimulated condition. CD3/CD28 antibody, SEB, IL27 and PMA plus ionomycin were valid stimulants for T cell activation.

CD69  /  interferon γ  /  lymphocyte proliferation  /  lymphocyte activation
Zheng YAN, Ni-na XUE, Ming JI, Fang-fang LAI, Xiao-guang CHEN. The effects of different stimulators on the activation of human lymphocytes[J]. Acta Pharmaceutica Sinica, 2019 , 54 (10) : 1863 -1867 . DOI: 10.16438/j.0513-4870.2019-0670
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Article Info
doi: 10.16438/j.0513-4870.2019-0670
  • Receive Date：2019-08-22
  • Online Date：2026-01-26
  • Published：2019-10-12
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  • Received：2019-08-22
  • Revised：2019-09-06
Funding
Affiliations
    State Key Laboratory of Bioactive Substances and Functions of Natural Medicines/Beijing Key Laboratory of Non-clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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