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The optimization and application of cell-based screening model for IL-17-mediated signaling pathway
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Ni-na XUE, Ming JI, Ming-yi ZHANG, Yi-chen LIU, Xiao-guang CHEN*
Acta Pharmaceutica Sinica | 2019, 54(10) : 1858 - 1862
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Acta Pharmaceutica Sinica | 2019, 54(10): 1858-1862
• Original Articles •
The optimization and application of cell-based screening model for IL-17-mediated signaling pathway
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Ni-na XUE, Ming JI, Ming-yi ZHANG, Yi-chen LIU, Xiao-guang CHEN*
Affiliations
  • State Key Laboratory of Bioactive Substances and Functions of Natural Medicines/Beijing Key Laboratory of Non-clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Published: 2019-10-12 doi: 10.16438/j.0513-4870.2019-0703
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We explore and verify the optimized condition for HEK-Blue IL-17 screening model, and screen the compounds that inhibits IL-17-medited signaling pathway. HEK-Blue IL-17 cells (5×104 cells per well) were seeded into the 96 plates followed by different concentrations of IL-17A or IL-17F alone, or in combination with tested compounds for 16 h. Then, the supernatant medium was incubated with QUANTI-Blue for 1 or 3 h to detect the OD value at λ655 nm. The secreted alkaline phosphatase (SEAP) production was an index of IL-17-mediated signaling activation in HEK-Blue IL-17 cells. We found that both IL-17A and IL-17F can significantly activate the IL-17 signaling pathway in HEK-Blue IL-17 cells. The available dosage of IL-17A and IL-17F were 10 and 100 ng·mL-1, respectively. The reaction time of SEAP and QUANTI-Blue was 1 h. In this model, arctigenin and epigallocatechin gallate (EGCG) could inhibit the IL-17A and IL-17F-mediated signaling pathway. This established and optimized screening model of HEK-Blue IL-17 cells was suitable for screening inhibitors of IL-17-mediated signaling pathway.

interleukin-17  /  screening model  /  autoimmune disease  /  tumor immunity
Ni-na XUE, Ming JI, Ming-yi ZHANG, Yi-chen LIU, Xiao-guang CHEN. The optimization and application of cell-based screening model for IL-17-mediated signaling pathway[J]. Acta Pharmaceutica Sinica, 2019 , 54 (10) : 1858 -1862 . DOI: 10.16438/j.0513-4870.2019-0703
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Article Info
doi: 10.16438/j.0513-4870.2019-0703
  • Receive Date：2019-08-30
  • Online Date：2026-01-26
  • Published：2019-10-12
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  • Received：2019-08-30
  • Revised：2019-09-23
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    State Key Laboratory of Bioactive Substances and Functions of Natural Medicines/Beijing Key Laboratory of Non-clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
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多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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