Home Journals Articles Updates About
CN
image
收藏切换
Comparison and theoretical modeling of capillary electrophoresis analysis for the interaction between caffeine and serum proteins
收藏切换
PDF
Qi-han XIA1, Ming GUO1, 2, *, Ping HOU1, Yi GU1, Fu-rong ZHAO1
Acta Pharmaceutica Sinica | 2019, 54(5) : 906 - 912
Less
收藏切换
Acta Pharmaceutica Sinica | 2019, 54(5): 906-912
• Original Articles •
Comparison and theoretical modeling of capillary electrophoresis analysis for the interaction between caffeine and serum proteins
Full
Qi-han XIA1, Ming GUO1, 2, *, Ping HOU1, Yi GU1, Fu-rong ZHAO1
Affiliations
  • 1. School of Forestry and Bio-technology, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
  • 2. School of Science, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
Published: 2019-05-12 doi: 10.16438/j.0513-4870.2018-1146
Outline
收藏切换

The method for analyzing the interaction between caffeine and human serum albumin (HSA) was established by capillary electrophoresis. Under physiological conditions, the interaction between ligand (caffeine)-receptor (HSA) was studied with frontier-analysis (FA) method, Hummel-Dreyer (HD) method and plug-plug kinetic (PPK) method. The interaction parameters of caffeine-HSA system were obtained using non-linear equation, Scatchard equation and Klotz equation. The results showed that FA, HD and PPK methods were suitable for caffeine-HSA system, among them, HD method was the best, and the Non-linear equation was the best theoretical model to caffeine-HSA system. Interaction parameter tests showed that caffeine-HSA interaction was a single site interaction and the binding stability was moderate. The mechanism of caffeine-HSA interaction has been elucidated, which can provide valuable information for further research of alkaloids.

alkaloid  /  caffeine  /  human serum albumin  /  theoretical model  /  interaction parameter
Qi-han XIA, Ming GUO, Ping HOU, Yi GU, Fu-rong ZHAO. Comparison and theoretical modeling of capillary electrophoresis analysis for the interaction between caffeine and serum proteins[J]. Acta Pharmaceutica Sinica, 2019 , 54 (5) : 906 -912 . DOI: 10.16438/j.0513-4870.2018-1146
Appendix
Less
Year 2019 volume 54 Issue 5
PDF
138
50
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2018-1146
  • Receive Date：2018-12-25
  • Online Date：2026-01-26
  • Published：2019-05-12
Article Data
Affiliations
History
  • Received：2018-12-25
  • Revised：2019-02-01
Funding
Affiliations
    1. School of Forestry and Bio-technology, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
    2. School of Science, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2018-1146
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House