Home Journals Articles Updates About
CN
image
收藏切换
Recent advances in enzyme inhibitor screening based on mass spectrometry
收藏切换
PDF
Li ZHU1, 2, Xuan ZOU1, 2, Tang-rong LIU1, 2, Ruo-nan LIU1, 2, Zhe XU1, 2, 3, *
Acta Pharmaceutica Sinica | 2019, 54(5) : 818 - 827
Less
收藏切换
Acta Pharmaceutica Sinica | 2019, 54(5): 818-827
• Reviews •
Recent advances in enzyme inhibitor screening based on mass spectrometry
Full
Li ZHU1, 2, Xuan ZOU1, 2, Tang-rong LIU1, 2, Ruo-nan LIU1, 2, Zhe XU1, 2, 3, *
Affiliations
  • 1. Key Laboratory of Marine Drugs, Chinese Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
  • 2. Laboratory for Marine Drugs and Bioproducts, Innovation Center for Marine Drugs Screening and Evaluation, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
  • 3. Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China
Published: 2019-05-12 doi: 10.16438/j.0513-4870.2018-1132
Outline
收藏切换

Enzymes play crucial functional roles in all biological processes. Enzymatic inhibitors can regulate enzyme activity and may become the starting point for drug discovery. Mass spectrometry (MS) has the advantage for rapid qualitative and quantitative analyses of compounds and enzyme reactions, emerging as an important analytical tool in enzyme inhibitor screening assay for drug discovery. This review will highlight recent advances in the inhibitor screening assay using MS and related techniques, including frontier affinity chromatography, immobilized enzyme beads, ultrafiltration, surface plasmon resonance, capillary electrophoresis and microfluidic chips. The existing MS methods for screening enzyme inhibitor were divided into two types:affinity screening and activity screening.

mass spectrometry  /  drug discovery  /  inhibitor screening  /  enzyme assay
Li ZHU, Xuan ZOU, Tang-rong LIU, Ruo-nan LIU, Zhe XU. Recent advances in enzyme inhibitor screening based on mass spectrometry[J]. Acta Pharmaceutica Sinica, 2019 , 54 (5) : 818 -827 . DOI: 10.16438/j.0513-4870.2018-1132
Appendix
Less
Year 2019 volume 54 Issue 5
PDF
133
53
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2018-1132
  • Receive Date：2018-12-21
  • Online Date：2026-01-26
  • Published：2019-05-12
Article Data
Affiliations
History
  • Received：2018-12-21
  • Revised：2019-01-24
Funding
Affiliations
    1. Key Laboratory of Marine Drugs, Chinese Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
    2. Laboratory for Marine Drugs and Bioproducts, Innovation Center for Marine Drugs Screening and Evaluation, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
    3. Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2018-1132
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House