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Design and expression of FGF23 inhibitor in Pichia pastoris
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Xiu-mei ZHANG1, Jia-jun CUI1, Yin-jie JIANG1, Nan-yan FU1, Jing YANG1, Zhi-xing ZHOU1, Ning-yuan ZHANG1, Kai QIAN1, 2, *
Acta Pharmaceutica Sinica | 2019, 54(2) : 380 - 385
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Acta Pharmaceutica Sinica | 2019, 54(2): 380-385
• Original Articles •
Design and expression of FGF23 inhibitor in Pichia pastoris
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Xiu-mei ZHANG1, Jia-jun CUI1, Yin-jie JIANG1, Nan-yan FU1, Jing YANG1, Zhi-xing ZHOU1, Ning-yuan ZHANG1, Kai QIAN1, 2, *
Affiliations
  • 1. Medical College, Yichun University, Yichun 336000, China
  • 2. Life Sciences College, Wenzhou University, Wenzhou 325000, China
Published: 2019-02-12 doi: 10.16438/j.0513-4870.2018-0959
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Hypophosphatemia is a common metabolism disease in humans. Fibroblast growth factor 23 (FGF23) inhibits phosphate reabsorption by targeting on the renal tubules. FGF23C-tail contains 73 amino acids from C-terminus of FGF23, serves as an inhibitor of FGF23, and can increase phosphate reabsorption. Therefore, FGF23C-tail is an important drug for hypophosphatemia. In this paper, we constructed a fusion protein of FGF23C-tail with HSA, and investigated the expression of the fusion protein in the Pichia pastoris system. The recombinant gene was constructed by fusion PCR. A high-yield strain was selected by G418 resistance and fermentation yield, and the expression yield was 43.7 mg·L-1 in flask. In 5 L fermenters, the highest expression yield could reach 265.6 mg·L-1. FGF23C-tail-HSA could be used as an inhibitor for FGF23, and could significantly increase blood phosphorus levels in rats. The procedures for care and use of animals were approved by the Ethics Committee of YiChun University. This paper provided a basis research for further studying physiological activity of FGF23C-tail-HSA.

FGF23  /  inhibitor  /  human serum albumin  /  hypophosphatemia
Xiu-mei ZHANG, Jia-jun CUI, Yin-jie JIANG, Nan-yan FU, Jing YANG, Zhi-xing ZHOU, Ning-yuan ZHANG, Kai QIAN. Design and expression of FGF23 inhibitor in Pichia pastoris[J]. Acta Pharmaceutica Sinica, 2019 , 54 (2) : 380 -385 . DOI: 10.16438/j.0513-4870.2018-0959
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Article Info
doi: 10.16438/j.0513-4870.2018-0959
  • Receive Date：2018-10-23
  • Online Date：2026-01-26
  • Published：2019-02-12
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  • Received：2018-10-23
  • Revised：2018-11-16
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    1. Medical College, Yichun University, Yichun 336000, China
    2. Life Sciences College, Wenzhou University, Wenzhou 325000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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