Home Journals Articles Updates About
CN
image
收藏切换
Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode
收藏切换
PDF
Ye-ting ZOU1, 2, Jin-di XU2, Fang LONG1, 3, Ye-qing ZHANG3, Song-lin LI1, 2, *
Acta Pharmaceutica Sinica | 2019, 54(1) : 130 - 137
Less
收藏切换
Acta Pharmaceutica Sinica | 2019, 54(1): 130-137
• ORIGINAL ARTICLES •
Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode
Full
Ye-ting ZOU1, 2, Jin-di XU2, Fang LONG1, 3, Ye-qing ZHANG3, Song-lin LI1, 2, *
Affiliations
  • 1. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
  • 2. Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of Chinese Academy of Traditional Chinese Medicine, Nanjing 210028, China
  • 3. Department of Respiratory Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
Published: 2019-01-12 doi: 10.16438/j.0513-4870.2018-0670
Outline
收藏切换

An ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) method was developed to evaluate the chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof. First, high resolution mass spectrometry data were obtained with Full scan mode, by comparing with MS data from the reference compounds and literatures, a total of 23 components were unequivocally or tentatively identified in ethanol extracts and acetic ether extracts thereof. Then, a mimic multiple reaction monitoring (mMRM) mode was established using UPLC-QTOF-MS/MS to quantify the triterpene acids in ethanol extracts and acetic ether extracts thereof. Eleven components were absolutely quantified with reference compounds, while 12 components without reference compounds were relatively quantified with peak areas, the transfer and enrichment rate of triterpene acids during liquid-liquid extraction were calculated. It was found all of the 23 triterpene acids identified in Poria ethanol extracts could be transferred into acetic ether extracts with high transfer and enrichment rate. The present study provides not only scientific evidence for further extraction of triterpene acids in Poria by acetic ether, but also an approach for comprehensive evaluation of the chemical consistency of herbal medicine extracts before and after the liquid-liquid extraction.

Poria  /  triterpene acid  /  liquid-liquid extraction  /  chemical consistency  /  UPLC-QTOF-MS/MS
Ye-ting ZOU, Jin-di XU, Fang LONG, Ye-qing ZHANG, Song-lin LI. Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode[J]. Acta Pharmaceutica Sinica, 2019 , 54 (1) : 130 -137 . DOI: 10.16438/j.0513-4870.2018-0670
Appendix
Less
Year 2019 volume 54 Issue 1
PDF
136
53
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2018-0670
  • Receive Date：2018-07-23
  • Online Date：2026-01-26
  • Published：2019-01-12
Article Data
Affiliations
History
  • Received：2018-07-23
  • Revised：2018-09-19
Funding
Affiliations
    1. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
    2. Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of Chinese Academy of Traditional Chinese Medicine, Nanjing 210028, China
    3. Department of Respiratory Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2018-0670
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House