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Identification of different Bupleurum varieties based on carbohydrate-specific chromatograms
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Li-xia SHI1, 2, Ke LI1, 2, 3, *, Xue-mei QIN1, 2, Zhen-yu LI1, 2, Lian-jie CUI1, 2, Shu-ying LI1, 2, Yu-xin CAO1, 2, Sheng-jin WANG4
Acta Pharmaceutica Sinica | 2020, 55(12) : 2968 - 2975
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Acta Pharmaceutica Sinica | 2020, 55(12): 2968-2975
• Original Articles •
Identification of different Bupleurum varieties based on carbohydrate-specific chromatograms
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Li-xia SHI1, 2, Ke LI1, 2, 3, *, Xue-mei QIN1, 2, Zhen-yu LI1, 2, Lian-jie CUI1, 2, Shu-ying LI1, 2, Yu-xin CAO1, 2, Sheng-jin WANG4
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
  • 2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China
  • 3. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
  • 4. Agricultural Comprehensive Development Center of Lingchuan County of Jincheng City of Shanxi Province, Jincheng 048300, China
Published: 2020-12-12 doi: 10.16438/j.0513-4870.2019-1054
Outline
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Characterization of the polysaccharides and monosaccharides of Bupleurum chinense was undertaken to identify differences in the Bupleurum chinense's sugar profiles, so as to provide a basis for the identification of different varieties. High performance liquid chromatography (HPLC) was used to generate chromatograms of the total polysaccharides of Bupleurum using an Evaporation Light Detector (ELSD), and a monosaccharide chromatogram was generated using a UV-detector (UV) following polysaccharide derivatization. The data were analyzed using SIMCA software and SPSS software to distinguish different varieties of Bupleurum. The results show that the yield of polysaccharides from Bupleurum falcatum is the highest, while the yield of polysaccharides from Bupleurum chinense is the lowest. The polysaccharide spectrum shows that the molecular weights of the polysaccharides in different Bupleurum differ, and their percentages of the total peak area are also different. The four Bupleurum polysaccharides are composed of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, and arabinose, but differ in length. The ratio of glucose to arabinose in Bupleurum chinense, Bupleurum scorzonerifolium, Bupleurum falcatum and Bupleurum marginatum var. stenophyllum is: 3.0-4.0, 5.5-7.0, 12.0-17.0, 9.0-12.0. In this study, a sugar profile technique was developed to provide a new method for the identification of different varieties of Bupleurum.

Bupleurum  /  polysaccharide  /  monosaccharide  /  characteristic spectrum  /  variety identification
Li-xia SHI, Ke LI, Xue-mei QIN, Zhen-yu LI, Lian-jie CUI, Shu-ying LI, Yu-xin CAO, Sheng-jin WANG. Identification of different Bupleurum varieties based on carbohydrate-specific chromatograms[J]. Acta Pharmaceutica Sinica, 2020 , 55 (12) : 2968 -2975 . DOI: 10.16438/j.0513-4870.2019-1054
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Article Info
doi: 10.16438/j.0513-4870.2019-1054
  • Receive Date：2020-05-24
  • Online Date：2026-01-23
  • Published：2020-12-12
Article Data
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History
  • Received：2020-05-24
  • Revised：2020-08-19
Funding
Affiliations
    1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
    2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China
    3. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
    4. Agricultural Comprehensive Development Center of Lingchuan County of Jincheng City of Shanxi Province, Jincheng 048300, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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