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The Cre-loxP system and its derivative systems:methodology research and applications in neuroscience
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Yang ZHANG1, Lin-tao JIA1, Yu-dong YAN2, Ya-nan ZHAO2, Yue-ming ZHANG2, Su-rong YANG2, *
Acta Pharmaceutica Sinica | 2020, 55(9) : 2035 - 2042
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Acta Pharmaceutica Sinica | 2020, 55(9): 2035-2042
• Reviews •
The Cre-loxP system and its derivative systems:methodology research and applications in neuroscience
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Yang ZHANG1, Lin-tao JIA1, Yu-dong YAN2, Ya-nan ZHAO2, Yue-ming ZHANG2, Su-rong YANG2, *
Affiliations
  • 1. School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
  • 2. Department of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
Published: 2020-09-12 doi: 10.16438/j.0513-4870.2020-0127
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In scientific research, it is often needed to knock in, knock out, knock down, or overexpress a specific gene in model organisms or specific types of cells to achieve precise regulation of experimental independent variables. In this case, various transgenic mice are required. The cyclization recombinase (Cre) can directly interact with different loxP (locus X over P1) DNA sequences without any cofactors to perform specific gene knock-in or knock-out at specific targets. Because of its advantages of simple action principles, high spatial specificity, and high reorganization efficiency, the Cre-loxP system is widely used in scientific research. Furthermore, the CreERT2 system (mutant of the fusion protein of Cre and estrogen receptor ligand binding domain) and the tetracycline (Tet)-on/off system, derived from the Cre-loxP system, have made the recombination of the target gene occur in temporal-specificity on the basis of spatial-specificity. This dual specificity of time and space is indispensable for research in specific directions such as fear memory and engram cells on the basis of reducing the impacts on experimental animals. Therefore, these derived systems have broad application prospects.

cyclization recombinase  /  CreERT2  /  Tet-on/off  /  optogenetics  /  chemogenetics
Yang ZHANG, Lin-tao JIA, Yu-dong YAN, Ya-nan ZHAO, Yue-ming ZHANG, Su-rong YANG. The Cre-loxP system and its derivative systems:methodology research and applications in neuroscience[J]. Acta Pharmaceutica Sinica, 2020 , 55 (9) : 2035 -2042 . DOI: 10.16438/j.0513-4870.2020-0127
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Article Info
doi: 10.16438/j.0513-4870.2020-0127
  • Receive Date：2020-02-14
  • Online Date：2026-01-23
  • Published：2020-09-12
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  • Received：2020-02-14
  • Revised：2020-03-28
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    1. School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
    2. Department of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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