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Gene cloning, subcellular localization and expression analysis of the AsERF1 gene from Aquilaria sinensis
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Tie-zheng LI1, Yi-zhe ZHENG1, Yu-qing RONG1, Sheng-li WEI2, Xiao-hui WANG1, 2, *, Peng-fei TU1, *
Acta Pharmaceutica Sinica | 2020, 55(8) : 1957 - 1964
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Acta Pharmaceutica Sinica | 2020, 55(8): 1957-1964
• Original Articles •
Gene cloning, subcellular localization and expression analysis of the AsERF1 gene from Aquilaria sinensis
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Tie-zheng LI1, Yi-zhe ZHENG1, Yu-qing RONG1, Sheng-li WEI2, Xiao-hui WANG1, 2, *, Peng-fei TU1, *
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
  • 2. Department of Resources and Identification of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
Published: 2020-08-12 doi: 10.16438/j.0513-4870.2020-0176
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Ethylene-response factors, which are a subfamily of the AP2/ERF family, play an important role in ethylene signal transduction, plant growth and plant resistant. In this study, a full-length cDNA of the AsERF1 gene was cloned from Aquilaria sinensis. Sequence analysis, prokaryotic expression and purification, subcellular localization, tissue-specific analysis and expression analysis under different abiotic stresses was performed. The open reading frame (ORF) of the AsERF1 gene was 691 bp, encoding a protein of 229 amino acids with a predicted molecular mass of 25.36 kD. The AsERF1 protein contained the conserved AP2 sequence of ERF protein. A phylogenetic analysis indicated that the AsERF1 protein showed greatest sequence similarity with ERF2 from Populus trichocarpa. The recombinant AsERF1 protein was expressed in Escherichia coli BL21(DE3) cells using the prokaryotic expression vector pET28a-AsERF1 and the recombinant AsERF1 protein was purified. Agrobacterium-mediated protein expression experiments demonstrated that AsERF1 mainly localized to the nucleus. Expression analysis indicated that AsERF1 was primarily observed in leaves. The AsERF1 expression level was induced by salt, drought, low temperature and CdCl2 treatment, while the abundance of AsERF1 was most significantly induced by drought stress. These results provide valuable insights into the role of AsERF1 in plant defense and the mechanism of agarwood formation.

Aquilaria sinensis  /  ethylene-response factors  /  prokaryotic expression  /  subcellular localization  /  expression analysis
Tie-zheng LI, Yi-zhe ZHENG, Yu-qing RONG, Sheng-li WEI, Xiao-hui WANG, Peng-fei TU. Gene cloning, subcellular localization and expression analysis of the AsERF1 gene from Aquilaria sinensis[J]. Acta Pharmaceutica Sinica, 2020 , 55 (8) : 1957 -1964 . DOI: 10.16438/j.0513-4870.2020-0176
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Article Info
doi: 10.16438/j.0513-4870.2020-0176
  • Receive Date：2020-02-25
  • Online Date：2026-01-21
  • Published：2020-08-12
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  • Received：2020-02-25
  • Revised：2020-03-13
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    1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
    2. Department of Resources and Identification of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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