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Evaluation of cultivated and wild Herba Epimedii using UHPLC-PDA-Q-TOF/MSE
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Ming ZHOU1, 2, 3, Wei ZHENG2, Bao-lin GUO3, *, An-jia CHEN1, *, Bai-ping MA2, *
Acta Pharmaceutica Sinica | 2020, 55(5) : 995 - 1003
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Acta Pharmaceutica Sinica | 2020, 55(5): 995-1003
• Original Articles •
Evaluation of cultivated and wild Herba Epimedii using UHPLC-PDA-Q-TOF/MSE
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Ming ZHOU1, 2, 3, Wei ZHENG2, Bao-lin GUO3, *, An-jia CHEN1, *, Bai-ping MA2, *
Affiliations
  • 1. Shanxi Medical University, Taiyuan 030001, China
  • 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
  • 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100193, China
Published: 2020-05-15 doi: 10.16438/j.0513-4870.2019-0885
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Qualitative and relatively quantitative methods were used to study the quality of cultivated Epimedium sagittatum (Sieb. et Zucc.) Maxim., Epimedium myrianthum Stearn, and Epimedium pubescens Maxim by ultra high-performance liquid chromatography coupled with photo-diode array and quadrupole time-of-flight mass spectrometry (UHPLC-PDA-Q-TOF/MSE). Thirty-two compounds in cultivated and wild samples of E. sagittatum, E. myrianthum and E. pubescens were identified using UHPLC-Q-TOF/MSE combined with the UNIFI data analysis platform. Principal component analysis (PCA) was used to compare the cultivated and wild samples of these three species. The results show that the chemical compositions of cultivated samples were consistent with the corresponding wild samples. UHPLC-PDA was used to determine the relative content of 12 flavonoids as well as total flavonoids in all samples. The results show that the relative chemical content of these flavonoids in cultivated and wild samples is similar and the quality of cultivated Epimedium is more stable. These qualitative and relatively quantitative methods using UHPLC-PDA-Q-TOF/MSE combined with the UNIFI data analysis platform and PCA can be used to study the quality of cultivated Herba Epimedii. This research provides a scientific basis for the cultivation and rational development and utilization of Epimedium medicinal materials.

Epimedium  /  cultivation  /  UHPLC-PDA-Q-TOF/MSE  /  quality study
Ming ZHOU, Wei ZHENG, Bao-lin GUO, An-jia CHEN, Bai-ping MA. Evaluation of cultivated and wild Herba Epimedii using UHPLC-PDA-Q-TOF/MSE[J]. Acta Pharmaceutica Sinica, 2020 , 55 (5) : 995 -1003 . DOI: 10.16438/j.0513-4870.2019-0885
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Article Info
doi: 10.16438/j.0513-4870.2019-0885
  • Receive Date：2019-11-07
  • Online Date：2026-01-21
  • Published：2020-05-15
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History
  • Received：2019-11-07
  • Revised：2019-12-29
Funding
Affiliations
    1. Shanxi Medical University, Taiyuan 030001, China
    2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
    3. Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100193, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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