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Matrix stiffness impacts the morphology and the levels of glucose transporter proteins in human renal tubular epithelial cells
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Xian-ru HAN, Yue-jing WANG, Tao GONG, Zhi-rong ZHANG, Yao FU*
Acta Pharmaceutica Sinica | 2018, 53(10) : 1736 - 1742
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Acta Pharmaceutica Sinica | 2018, 53(10): 1736-1742
• Pharmaceutics •
Matrix stiffness impacts the morphology and the levels of glucose transporter proteins in human renal tubular epithelial cells
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Xian-ru HAN, Yue-jing WANG, Tao GONG, Zhi-rong ZHANG, Yao FU*
Affiliations
  • Key Laboratory of Drug Targeting and Delivery System, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China
Published: 2018-10-12 doi: 10.16438/j.0513-4870.2018-0586
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As the common pathway of chronic renal diseases leading to end-stage renal failure, renal tubulointerstitial fibrosis is characterized by the deposition of extracellular matrix and scar hardening. Our study aimed to construct an in vitro cell culture platform to explore the impact of matrix stiffness on cell morphology and function of renal tubular epithelial cells. Photopolymerized polyacrylamide gels (PAA gel) with varying stiffnesses as model substrates was selected to simulate the matrix stiffness of normal and fibrotic renal tissues with elastic moduli ranging from 1 to 40 kPa. The human renal tubular epithelial cells (HK-2) were seeded on the surface of PAA gels. The impact of matrix stiffness on the morphology of HK-2 were investigated via immunofluorescence staining and confocal microscopy. The expression levels of glucose transporter 1 (GLUT1), glucose transporter 2 (GLUT2), glucose transporter 5 (GLUT5) were semi-quantitatively analyzed. With increasing matrix stiffness, both the levels of GLUT1 and GLUT5 in HK-2 cells were significantly decreased, whereas the expression level and the distribution pattern of GLUT2 in HK-2 remained unchanged with stiffness variation.

matrix stiffness  /  renal tubular epithelial cell  /  cell morphology  /  glucose transporter 1  /  glucose transporter 2  /  glucose transporter 5
Xian-ru HAN, Yue-jing WANG, Tao GONG, Zhi-rong ZHANG, Yao FU. Matrix stiffness impacts the morphology and the levels of glucose transporter proteins in human renal tubular epithelial cells[J]. Acta Pharmaceutica Sinica, 2018 , 53 (10) : 1736 -1742 . DOI: 10.16438/j.0513-4870.2018-0586
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doi: 10.16438/j.0513-4870.2018-0586
  • Receive Date：2018-06-25
  • Online Date：2026-01-15
  • Published：2018-10-12
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  • Received：2018-06-25
  • Revised：2018-07-06
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    Key Laboratory of Drug Targeting and Delivery System, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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