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Establishment of a cell-based evaluation system for arenavirus entry inhibitor
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Xiao-yu ZHANG, Ke TANG, Jia-mei GUO, Qing CHEN, Ying GUO*
Acta Pharmaceutica Sinica | 2018, 53(5) : 735 - 742
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Acta Pharmaceutica Sinica | 2018, 53(5): 735-742
• ORIGINAL ARTICLES·Pharmacology •
Establishment of a cell-based evaluation system for arenavirus entry inhibitor
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Xiao-yu ZHANG, Ke TANG, Jia-mei GUO, Qing CHEN, Ying GUO*
Affiliations
  • Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China
Published: 2018-05-12 doi: 10.16438/j.0513-4870.2018-0028
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Arenaviruses are enveloped RNA viruses. The genus mammarenavirus contains nine members that are known to be human pathogens, and eight of them cause human hemorrhagic fever. Lassa hemorrhagic fever, caused by Lassa virus (LASV) infection, is the most prevalent arenavirus hemorrhagic fever with potential to cause major epidemics. LASV belongs to category A agents, and biosafety level-4 (BSL-4) facility is required for live virus experiments. Currently there are few specific treatments available for arenavirus diseases. Here, we established efficient cell-based pseudovirus infection models using an HIV-1 core (pNL4-3.Luc.R-E-) packed with arenavirus glycoproteins. Nine recombinant arenaviruses (arenavirus-GP/HIV-luc) were generated, and 17 cell lines were tested for susceptibilities to these viruses. These pseudovirus infection models were further validated by known arenavirus entry inhibitors. The models are safe and specific to pseudovirus infection, which are readily used for pharmacodynamic evaluation of arenavirus entry inhibitors in BSL-2 laboratory. The models will facilitate screening of the anti-arenavirus drugs and vaccines.

arenavirus  /  viral hemorrhagic fever  /  pseudovirus  /  entry inhibitor
Xiao-yu ZHANG, Ke TANG, Jia-mei GUO, Qing CHEN, Ying GUO. Establishment of a cell-based evaluation system for arenavirus entry inhibitor[J]. Acta Pharmaceutica Sinica, 2018 , 53 (5) : 735 -742 . DOI: 10.16438/j.0513-4870.2018-0028
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doi: 10.16438/j.0513-4870.2018-0028
  • Receive Date：2018-01-08
  • Online Date：2026-01-15
  • Published：2018-05-12
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  • Received：2018-01-08
  • Revised：2018-01-29
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    Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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